Live/dead stain for embryos and oocytes FDAÓ«¹âËضþÒÒËáõ¥ºÍEBä廯ÒÒ¶§È¾É«Çø·ÖÅßÌ

Procedures obtained from Ann Croy (Guelph, Canada) as a modification of procedures detailed in Transplantation 1971 12:148-151 Takasugi, M. An improved fluorochromatic cytotoxic test. Note that the procedure can be used for staining of all cells and not just embryos or oocytes. Also, ethididium bromide can be replaced with propidium iodide, DAPI or Hoescht 33342

Materials

Ethidium Bromide (EtBr; Fisher)

Fluorescein Diacetate (FDA; Sigma)

DPBS

Acetone

Procedure

1) Make following stock solutions:

EtBr (10 mg/mL DPBS) Store in dark at 4 C

FDA (5 mg/mL acetone) Store in dark in glass container at -20 C

Storage life of stocks ~4 months

2)

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