The Preuss Lab,The Division of Biological Sciences,The University of Chicago
http://preuss.bsd.uchicago.edu/protocols/pgmedia.html
0.01% boric acid
1 mM MgSO4
2mM CaCl2--this was used with media containing GABA. If no other nitrogen source is present, use 1mM CaCl2 and 1 mM CaNO3
18% sucrose
pH 6.5
Air dry flowers for 1-2 hours (optional--this helps with release from anthers but is not essential), then dab anthers onto surface of a 25-30µL drop of germination media. Drop can be on a depression slide or on a coated slide designed to keep samples separate. Use 1 flower per drop. Invert and incubate overnight in 100% humidity. Then place small drops of vacuum grease on the slide and gently place a coverslip on top. Allow the coverslip to just touch the drops of liquid but not to squish them. Hicks et al. Plant Phys 134: 1227-39. 2004
上一篇：Co-staining with Thioflavin S 下一篇：MS Plant Tissue Culture Medium [University of Zurich] |
