I usually use Linear polyacrylamide as a carrier for DNA precipitation by EtOH. I have read about using glycogen as a carrier, I would like to know if anyone knows a method avoiding not to use acrylamide (for which legislation is hard in France) as carrier for DNA.
Thanks for all -ragno-
Hello,
I know for the french legislation, i work in french laboratory. As carrier you can use glycogen at 10 micro or plus when you precipitate with EtOH. You can also put microtube at -80癈 15mn or overnight at -20癈 when small amount DNA. Glycogen is put into tube before Na acetate+EtOH. You can also use yeast tRNA as carrier, it's work well.
michel -mariet-
Are you sure you are not confusing POLYacrylamide and acrylamide (the monomer). LPA (Linear Poly Acrylamide) can be purchased cheaply from Sigma and used as a carrier. --
You can also use dextran blue. --
I have searced in Sigma, but I have not found LPA.Where could I find it (is it solid or liquid)?Thank you for all -mariet-
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