I am working with very small amount human tissue. Is there anyone who had the experience of using glycogen with 100% ethanol in pheno/chloroform DNA extraction protocol ? Does it realy work to increase DNA yield much?
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The glycogen is just there as a co-precipitant to ensure that you do not lose your pellet and won't do anything to your 260/280 readings. If you are really worried about this step then Ambion do a product called glyco blue which makes it virtually impossible to lose the pellet.
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I have used glycogen ( about 2 micrograms ) for precipitating In vitro transcribed RNA.sankar
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