| Transgenic Mouse and Gene Targeting Facility
Tg 008 Southern Blotting
Materials and Equipment
Hybaid hybridization oven (ISC BioExpress, H-9250)
Vacuum oven (VWR, 52201-218)
Ludlum Geiger Counter
Radiation Shields and lock box (from various vendors)
Mini-centrifuge (VWR, 20668-212)
Thermolyne Dri-Bath, Type 17600 (VWR, 35756-006)
Platform Shaker, New Brunswick Scientific (VWR, 40000-300)
Electrophoresis Separation Systems (Owl)
QIAquick Nucleotide Removal Kit (Qiagen, 28304)
Prime-It II Random Primer Labeling Kit (Stratagene, 300385)
[alpha-32P]dCTP (ICN)
Salmon Sperm DNA (Stratagene, 201190)
SDS solution, 20% w/v (Bio-Rad, 161-0418)
Zeta-Probe Genomic Tested Blotting Membrane (nylon) (Bio-Rad, 162-0196)
Gel Blot Paper GB002 (Schleicher
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