| Cepko/Tabin Lab ,Harvard University http://axon.med.harvard.edu/~cepko/protocol/mike/S6.html
Superfrost plus slides and standard slides can be silinized but I have found that superfrost slides work best so that protocol is outlined here.
Solutions
Reagents Needed
Superfrost Plus Slides
TESPA-3 aminopropyltrioxysilane (Sigma # A3648)
acetone
EtOH
Concentrated HCl
Procedure
• Dip a rack of superfrost plus slides in 2N HCl (200 ml Q 40 ml concentrated HCl) for 1 minute.
• Rinse the slides for 1 minute in Milli Q water.
• Rinse the slides in 100% Acetone for 1 minute.
• Incubate in 100% Acetone containing TESPA for 1 minute.
• Rinse briefly in 100% acetone.
• Rinse briefly in 95% EtOH followed by 50% EtOH, 30% EtOH and several changes of Q.
• Air dry at room temperature overnight and then store at 4° dessicated until use. They keep for several months.
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