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FIGURE 1. Movement of mRNA on the ribosome
a, b, tRNA–mRNA interaction in the 70S initiation (a) and post-initiation (b) complexes. (E-tRNA was co-purified with the ribosome.) c, Electron-density difference map between the SD(U)9UUU(U)4 post-initiation and the SD(A)4AUG(A)9 initiation complexes. Positive differences are in grey; negative differences are in red. d, Movement of mRNA from initiation (yellow) to post-initiation (grey) gives rise to a simultaneous positional rearrangement and clockwise rotation of the SD duplex. e, f, Detailed view of the ribosomal environment of the SD duplex in the initiation (e) and post-initiation (f) complexes.
生物谷报道：转位是核糖体功能的最重要特征之一，它对于一个基因的表达水平具有重要的影响。而此前，一直没有发现这个过程中关于mRNA运动的结构信息，即在翻译的不同阶段，mRNA是怎样和核糖体相互作用的呢？
现在，Yusupova等科研人员描述了当从初始状态转变为后初始状态时在一个3'-5'方向上的mRNA转动和运动。这是对mRNA动态的首次实验观测，它对了解转位机制将具有广泛的意义，该结果发表在最新的一期《Nature》杂志上。
原文出处：
Nature Volume 444 Number 7117
Structural basis for messenger RNA movement on the ribosome
Gulnara Yusupova, Lasse Jenner, Bernard Rees, Dino Moras and Marat Yusupov
全文下载：
First paragraph | Full Text | PDF (861K) | Supplementary information
See also: Editor's summary
作者简介：
YUSUPOVA Gulnara - Ingénieur de recherche
Office: ESBS
Personal Section: Link to the personal page:
http://www-bio3d-igbmc.u-strasbg.fr/~gula
About the Lab:
The Structural Biology and Genomics Laboratory (SBGL) is located near Strasbourg, in the north/east part of France (Alsace) close to the German border. The Lab. has been directed by Dr Dino Moras since its creation. The laboratory was created in 1980 and was originally named the "Laboratoire de Biologie Structurale" (Structural Biology Laboratory). It was then the fifth department of the IBMC (Institut de Biologie Moléculaire et Cellulaire), in downtown Strasbourg. The laboratory was aimed at the understanding of the molecular mechanism responsible for the fidelity of the aminoacylation reaction of transfer-RNA. More recently (1990), in a collaboration with the laboratory of Prof P. Chambon, the laboratory initiated a new project in the nuclear receptor (NR) field. At the end of 1994, we moved to the IGBMC (Institut de Génétique et de Biologie Moléculaire et Cellulaire). The laboratory's current focus is the study of the structure/function relationship of macromolecules, employing biophysical means to solve the 3D structure (Xray cristallography, Nuclear Magnetic Resonance and, in collaboration, Electron Microscopy). The scientific activities of the laboratory can be divided in four major themes :
- Translation, with special emphasis on aminoacyl-tRNA synthetases and transfer RNA
- DNA structure and Transcription. A major project of the laboratory is currently the structural investigation of nuclear receptors an their functional implications in activation and regulation of transcription. A new project has been started recently and concerns the structural investigation of the general transcription factor TFIIH
- Proteins of therapeutical importance. Recently the structure of a membrane receptor has been elucidated FhuA (ferric hydroxamate uptake protein A), as well as a matrix metallo-protease
- Genomics and bioinformatics plays a transversal role in the scientific activity of the laboratory and the laboratory has numerous ongoing projects in this field
Publications:
Pubmed
相关报道：
mRNA顺式作用元件调节翻译控制
通过磷酸化elF2或elF2B调节mRNA翻译
通过mTOR信号途径的下游靶调节mRNA翻译
研究发现转录后mRNA表达调控的新机制
第十二节：翻译：蛋白质的生物合成——rRNA和核糖体
《Cell》：核糖体研究新发现
核糖体与新生肽的保护
核糖体高度保守位点和蛋白合成无关
上一篇：李龙承：反标准的新RNAi过程 下一篇：研究发现12个microRNA与心脏病有关
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