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生物谷报道：另类拼接过程的存在，能使一个基因产生多种蛋白。现在，一种新型RNA图被绘制出来，该RNA图可以反映短的RNA片段是怎样影响另类拼接过程在大脑中被控制的方式的。这一成果被发表于最新一期的《Nature》期刊上。
研究人员知道，另类拼接过程为大脑中所发生的过程增添一层复杂性，但它到底是怎样被调控的（有时会失控而引起疾病）却仍然不清楚。该新型RNA图将生物信息学、生物化学和遗传学方法结合起来确定一组规则，按照这些规则，Nova基因调控小鼠体内神经元特有的RNA结合蛋白的拼接增强子或沉寂子的活性。这些结果有助于我们了解学习和记忆等大脑功能以及神经疾病和癌症生物学。
FIGURE 1. Definition of the Nova–RNA binding map.
a, A generic pre-mRNA showing the four regions that define the Nova–RNA binding map (the start and end of each region is labelled by a nucleotide distance to the splice site). Peaks demonstrate the positions of Nova-dependent splicing enhancers (red) or silencers (blue). b, A conserved YCAY cluster score (y axis) was calculated as described in Supplementary Methods. The x axis shows the nucleotide position of the centre of the sequence window. At each significant peak of YCAY cluster enrichment in Nova-regulated versus control pre-mRNAs, the P-value (two-tailed t-test, unequal variance) of YCAY clusters is shown. The error value of the control pre-mRNAs represents standard deviation of the mean values of 100 random groups of 20 control pre-mRNAs. c, YCAY cluster distribution in eight Nova-regulated pre-mRNAs that contain both NISE2 and NISE3 elements.
原文出处：
Nature Volume 444 Number 7119
An RNA map predicting Nova-dependent splicing regulation p580
Jernej Ule, Giovanni Stefani, Aldo Mele, Matteo Ruggiu, Xuning Wang, Bahar Taneri, Terry Gaasterland, Benjamin J. Blencowe and Robert B. Darnell
doi:10.1038/nature05304
Abstract | Full Text | PDF (1,073K) | Supplementary information
See also: Editor's summary
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上一篇：蔡南海院士：miRNA研究新发现 下一篇：PNAS：一个致癌microRNA的激活
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