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Protocol for mRNA amplification，非常详细的RT-PCR实验过程[Howard Hughes Medical

点击：   作者：51protocol收集   来源：  时间： 2007-03-19 　本站论坛

42C for 90min in thermal cycler.

Second strand synthesis:

Add to PCR Tube

Ammt

Reagent

106 ul

DEPC H2O

15ul

Advantage PCR buffer

3ul

10 mM dNTP mix

1ul

RNase H (2U/ul Gibco BRL Cat# 18021-071)

3ul

Advantage Polymerase (Clontech Cat# 8417-1)


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