(2). G/C-rich 3' ends may be too sticky and give non-specific products. Using this additional rule primer length might increase to 25 or 26 bases. We have not yet used this rule in our primer-picking but we are experimenting.
REFERENCES
1. Cheng, S., Fockler, C., Barnes, W., Higuchi, R.
Effective amplification of long targets from cloned inserts and human genomic DNA. Proc. Natl. Acad. Sci. 91, 5695-5699 (1994).
2. Crameri, A. and Stemmer, W. 10^20-Fold aptamer library amplification without gel purification. Nucleic Acids Research 21, 4410 (1993)
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