Note: Samples for a standard curve should be included in EVERY real-time run for quantitation.
Note: Negative controls for every primer set used should be included to verify the absence of primer dimer amplification and contamination.
5.3 Thermal Cycling Parameters
5.3.1 For Qiagen QuantiTect SYBR Green PCR Kit
Dissociation/Melting Curve (machine specific)
5.3.2 For Applied Biosystems SYBR Green PCR Master Mix:
上一篇：RT-PCR简介 下一篇：Rt-PCR实验步骤
共2页: 上一页 [1] 2 下一页
