A comb is inserted into the top of the gel to create the wells into which the samples will be loaded.
Once the gel is set, the unit is placed into the sequencing machine.
Samples are taken up with a multi-pipettor …
…and loaded into the wells of the gel.
This is a close-up from the previous photograph. Note the extremely small size of the wells and the pipettor, making it possible to load large numbers of samples into each gel.
A heating plate is placed against the gel to ensure a constant performing temperature.
Gel buffer is loaded into the top tank…
…and into the bottom tank.
A final look, then the door is shut and the program begun.
In summary
! PCR is a simple technique to obtain many copies of specific DNA fragments
! Three steps are involved in PCR: denaturation,annealing and extension
! To ensure success, care should be taken both in preparing the reaction mixture and setting up the cycling conditions
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