我们用一系列样品检测了多个参数来优化多重 PCR 。对于任何 PCR 反应，为了获得高特异性的扩增产物，退火温度和 KCl 浓度（盐浓度）的最优组合是最重要的。 Mgcl 2 浓度应当与 dNTP 的量成比例，对于任何反应这个比值可以是常数。虽然逐渐提高 MgCL 2 浓度可以增强反应的特异性，但是看起来退火温度和 KCl 浓度（盐浓度）对于获得高产量的特异性 PCR 扩增产物更为重要。在多重 PCR 中调节每一个基因的引物量也很重要。 Figure 1 给出了进行高效的多重 PCR 的有效方法。尽管无法完全列出影响 PCR 反应的所有因素。然而本研究中所提到的参数的优化提供了解决多重 PCR 中常见问题的基本方法。
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