1% sodium deoxycholic acid (deoxycholate, sodium salt) 1.0 gram
*10 mM DTT 0.154 grams
*1% IGEPAL CA-630 (Nonidet P-40, NP-40) 1.0 mL
*2% (w/v) PVP-40 2.0 grams
* = add just before use. To dissolve, add Borax, EGTA, SDS, and sodium deoxycholic acid to pre-warmed deionized, distilled water. Adjust pH to 9.0 with sodium hydroxide. DEPC-treat and autoclave.
For 38ml aliquot of XT Buffer:
DTT 0.6g
PVP 0.73g
IGEPAL 360µl
-
Proteinase K (10 mg/mL in DEPC-treated dd-water; store stock at -20°C)
-
1X TE (10 mM Tris-Hcl, pH 7.5 with 1 mM EDTA, pH 8.0)
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2 M Potassium chloride
-
2 M Potassium acetate, pH 5.5
-
2 mM AND 8 mM Lithium chloride (keep in refrigerator)
-
3 M Sodium acetate, pH 6.0
-
Ethanol, 70% and 100% (keep at -20°C)
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Liquid nitrogen (for tissue grinding)
