目前已发表文章中RNAi设计的片段综述
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Gene (Ref)

Accession #

Position from ATG

Gene Target
(Comments)

Cell Line Tested

NM_001101

972-994

aatgaagatcaagatcattgc

Human HeLa

NM_001614

8-30

aagagatcgccgcgctggtca

Human HeLa
Ishikawa
978-998
AATTGGTGCACTTTTACGTGA
IMR90E1

AF006086

181-203

aagaatgaagctgataggacc

Human HeLa
AF451323
254-274
AAGGTCCACAGATTATTAGAT
Human HeLa
AF451323
464-484
CTAGAGGAACAGAGAAGATCACA
Human HeLa
AF451323
867-887
AAGAAGAGGCCCAGAAAAGCT
Human HeLa
AAGCAGAGUUCAAAAGCCCUU
AAAGCAGAGUUCAAAAGCCCU
AAGACAGAGCCAAGUGGACUC
Mouse P19
1682-1700
AAUUGGUAAACGAUCAAGGAG
RBL-2H3, WEHI-231, NALM-6
895-913
AAGGGAAAGAAGGAGGUUUCA
RBL-2H3, WEHI-231, NALM-6
518-536
AAGAAGCUUAAAACCUGGGAG
RBL-2H3, WEHI-231, NALM-6
 
520-538
AACUGGGGAAGAAGGGCAACC
HEK293
191-211
AAGGGGCACAGGCATGCCAAA
IMR90E1A
94-114
AAACAGCTGTTGTTGAGCGAA
IMR90E1A
125-147
HeLa
89-109
GCACAGTAAATACCCACTATT
HeLa

NM_001786

125-147

aaggggttcctagtactgcaa

Human HeLa

NM_001813

944-966

aaactcttactgctctccagt

Human HeLa
 
138-156
AAGGUCAGUAAUACAACGGUG
CHO
  
 
AACCACCGCCCUCCGUGCA
HL-5 and 293
J03166
199-217
CTCGTACAGCCTTGGGAGACA
Xenopus embryo
J03167
252-270
ATGTTAAGACTAAGCATGTAC
Xenopus embryo

U53530

509-531

aaggccaaggaggcgctggaa

Human HeLa
DLD1
DLD1
Dishevelled (hDv-1 and hDv-3) (16)
 
 
 
AACAAGATCACCTTCTCCGAG
A293
 
hDv-2 (16)
 
 
 
AACTTTGAGAACATGAGCAAC
A293
 

NM_004523

1547-1569

aactggatcgtaagaaggcag

Human HeLa

NM_000117

628-650

aaccgtgctcctggggctggg

Human HeLa
 
 
AACUCCUUGAACGGCAGCCGC
Drosophila embryos
713-735
see reference
HeLa
121-141
AAGGUAACAUUGAUGACUCGC
U2OS
65-87
HeLa
 
461-482
HeLa

NM_006530

327-349

aagacctgtaaccctgtatca

Human HeLa
1517-1539
HeLa-S3
AAGAGGUAUAUGACUUUGCUU
CaSKi, SiHa, NDF, HCT116
AAAGGAGGAUGAAAUAGAUGG
U2OS
U2OS

NM_000224

1154-1176

aagatggcgaggactttaatc

Human HeLa
160-180
AAGCAGTTCTTGCCGCCAGGA
HEK293
160-180
AAGCAGTTCCTGCCTCGGGGA
HEK293
NM019390
 
260-280
GAAGCAGCUUCAGGAUGAGAU
Human HeLa S3
 
 
AAGGACCUGGAGGUCUGCUG
Hep3B

NM_005572

608-630

aactggacttccagaagaaca
(90% reduction; 2)

Human HeLa

NM_005573

672-694

aacgcgcttggtagaggtgga

Human HeLa

M94362

1457-1479

aagaggaggaggaagccgagt

Human HeLa

NM_003276

37-59

aaagacaagttgaagagtgag

Human HeLa
AF083811
1843-1865
See reference
HeLa-S3
AF189712
472-492
See reference
HeLa
Methyl-CpG-binding protein 2 (MeCP2) (19)
 
 
 
AAGCAUGAGCCCGUGCAGCCA
Hep3B
 
 
AAGAGGAUGGAUUGCCCGGCC
Hep3B
AAUCCUUCGUCUUUCCUCUCG
primary neuronal cells
45-63
See reference
HeLa, MycB
 
133-155
See reference
Human HeLa S3
456-476
AACCCGUCCGUACUGGUGUUA
U2OS
AAGGGCAAGTTTCCCGTGCAG
HEK293

Z11583

3988-4010

aaggcgtggcaggagaagttc

Human HeLa

NM_005124

2593-2615

aaggcagactctaccaaatgt

Human HeLa
AAUGGUAGCCACAUCCGGUGU
HeLa
AAGATTGGACAGCAGGUCGAC
HeLa
566-584; 639-657; 1958-1976; 2780-2798
See reference
181-203
AAGGGCGGCTTTGCCAAGTGC
HeLa, U2OS
 
931-949
AAGATCAAGTTTTGCCAATG
Human HeLa
U53174
232-252
MCF-7
 
 
AACAGACUGGGUUGACCCAUC
Human HeLa
HSU73377
677-697
tcctacttggttcggtacatggg
Human HeLa
HSU73377
236-256
CGGAATGAGTCTCTGTCATCGTC
Human HeLa
Sphingosine kinase 1 (hSK1) (15)
 
 
70 nt downstream of start codon
 
AAGAGCUGCAAGGCCUUGCCC
HUVEC
Ishikawa
AF312873
3'-UTR
GACAGAGCCAAGUGGACUCAC
Mouse P19
M16553
 
 
AAGCGCUUCAGGCACUACAAA
HeLa, 293, Cos-1, HaCaT
 
M16553
 
 
AAGAAGCAGACGUACUUGGCA
HeLa, 293, Cos-1, HaCaT
 
 
 
GUGGAAUUUGUGGAACAUCTT
293
 
413-433
AACCUCCAGUCUUCUCUCGUC
Human HeLa-M & HeLa KK3

XM_009141

1087-1109

aaagaggaaatcattgaagcc

Human HeLa

NM_003380

346-368

aactacatcgacaaggtgcgc
(No silencing observed; 1,2)

Human HeLa

NM_003380

1145-1167

aataccaagacctgctcaatg

Human HeLa

NM_003380

863-885

aagaatggtacaaatccaagt

Human HeLa

NM_003380

1037-1059

aaatggaagagaactttgccg

Human HeLa

L18880

2923-2945

aagcggattagaaccaatctc

Mouse 3T3

X99063

1357-1379

aaagcctaccacccacagtgc

Mouse 3T3
 

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声明:仅到2003末为止,2004以后这样的文章更多,大家可以搜索pubmed。

 

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