目前已发表文章中RNAi设计的片段综述- 点击: 作者: 来源: 日期:2006-11-07 本站论坛
声明:仅到2003末为止,2004以后这样的文章更多,大家可以搜索pubmed。
声明:仅到2003末为止,2004以后这样的文章更多,大家可以搜索pubmed。
Gene (Ref)
Accession #
Position from ATG
Gene Target (Comments)
Cell Line Tested
NM_001101
972-994
aatgaagatcaagatcattgc
Human HeLa
NM_001614
8-30
aagagatcgccgcgctggtca
Human HeLa
Ishikawa
978-998
AATTGGTGCACTTTTACGTGA
IMR90E1
AF006086
181-203
aagaatgaagctgataggacc
Human HeLa
AF451323
254-274
AAGGTCCACAGATTATTAGAT
Human HeLa
AF451323
464-484
CTAGAGGAACAGAGAAGATCACA
Human HeLa
AF451323
867-887
AAGAAGAGGCCCAGAAAAGCT
Human HeLa
AAGCAGAGUUCAAAAGCCCUU
AAAGCAGAGUUCAAAAGCCCU
AAGACAGAGCCAAGUGGACUC
Mouse P19
1682-1700
AAUUGGUAAACGAUCAAGGAG
RBL-2H3, WEHI-231, NALM-6
895-913
AAGGGAAAGAAGGAGGUUUCA
RBL-2H3, WEHI-231, NALM-6
518-536
AAGAAGCUUAAAACCUGGGAG
RBL-2H3, WEHI-231, NALM-6
520-538
AACUGGGGAAGAAGGGCAACC
HEK293
191-211
AAGGGGCACAGGCATGCCAAA
IMR90E1A
94-114
AAACAGCTGTTGTTGAGCGAA
IMR90E1A
125-147
HeLa
89-109
GCACAGTAAATACCCACTATT
HeLa
NM_001786
125-147
aaggggttcctagtactgcaa
Human HeLa
NM_001813
944-966
aaactcttactgctctccagt
Human HeLa
138-156
AAGGUCAGUAAUACAACGGUG
CHO
AACCACCGCCCUCCGUGCA
HL-5 and 293
J03166
199-217
CTCGTACAGCCTTGGGAGACA
Xenopus embryo
J03167
252-270
ATGTTAAGACTAAGCATGTAC
Xenopus embryo
U53530
509-531
aaggccaaggaggcgctggaa
Human HeLa
DLD1
DLD1
Dishevelled (hDv-1 and hDv-3) (16)
AACAAGATCACCTTCTCCGAG
A293
hDv-2 (16)
AACTTTGAGAACATGAGCAAC
A293
NM_004523
1547-1569
aactggatcgtaagaaggcag
Human HeLa
NM_000117
628-650
aaccgtgctcctggggctggg
Human HeLa
AACUCCUUGAACGGCAGCCGC
Drosophila embryos
713-735
see reference
HeLa
121-141
AAGGUAACAUUGAUGACUCGC
U2OS
65-87
HeLa
461-482
HeLa
NM_006530
327-349
aagacctgtaaccctgtatca
Human HeLa
1517-1539
HeLa-S3
AAGAGGUAUAUGACUUUGCUU
CaSKi, SiHa, NDF, HCT116
AAAGGAGGAUGAAAUAGAUGG
U2OS
U2OS
NM_000224
1154-1176
aagatggcgaggactttaatc
Human HeLa
160-180
AAGCAGTTCTTGCCGCCAGGA
HEK293
160-180
AAGCAGTTCCTGCCTCGGGGA
HEK293
NM019390
260-280
GAAGCAGCUUCAGGAUGAGAU
Human HeLa S3
AAGGACCUGGAGGUCUGCUG
Hep3B
NM_005572
608-630
aactggacttccagaagaaca (90% reduction; 2)
Human HeLa
NM_005573
672-694
aacgcgcttggtagaggtgga
Human HeLa
M94362
1457-1479
aagaggaggaggaagccgagt
Human HeLa
NM_003276
37-59
aaagacaagttgaagagtgag
Human HeLa
AF083811
1843-1865
See reference
HeLa-S3
AF189712
472-492
See reference
HeLa
Methyl-CpG-binding protein 2 (MeCP2) (19)
AAGCAUGAGCCCGUGCAGCCA
Hep3B
AAGAGGAUGGAUUGCCCGGCC
Hep3B
AAUCCUUCGUCUUUCCUCUCG
primary neuronal cells
45-63
See reference
HeLa, MycB
133-155
See reference
Human HeLa S3
456-476
AACCCGUCCGUACUGGUGUUA
U2OS
AAGGGCAAGTTTCCCGTGCAG
HEK293
Z11583
3988-4010
aaggcgtggcaggagaagttc
Human HeLa
NM_005124
2593-2615
aaggcagactctaccaaatgt
Human HeLa
AAUGGUAGCCACAUCCGGUGU
HeLa
AAGATTGGACAGCAGGUCGAC
HeLa
566-584; 639-657; 1958-1976; 2780-2798
See reference
181-203
AAGGGCGGCTTTGCCAAGTGC
HeLa, U2OS
931-949
AAGATCAAGTTTTGCCAATG
Human HeLa
U53174
232-252
MCF-7
AACAGACUGGGUUGACCCAUC
Human HeLa
HSU73377
677-697
tcctacttggttcggtacatggg
Human HeLa
HSU73377
236-256
CGGAATGAGTCTCTGTCATCGTC
Human HeLa
Sphingosine kinase 1 (hSK1) (15)
70 nt downstream of start codon
AAGAGCUGCAAGGCCUUGCCC
HUVEC
Ishikawa
AF312873
3'-UTR
GACAGAGCCAAGUGGACUCAC
Mouse P19
M16553
AAGCGCUUCAGGCACUACAAA
HeLa, 293, Cos-1, HaCaT
M16553
AAGAAGCAGACGUACUUGGCA
HeLa, 293, Cos-1, HaCaT
GUGGAAUUUGUGGAACAUCTT
293
413-433
AACCUCCAGUCUUCUCUCGUC
Human HeLa-M & HeLa KK3
XM_009141
1087-1109
aaagaggaaatcattgaagcc
Human HeLa
NM_003380
346-368
aactacatcgacaaggtgcgc (No silencing observed; 1,2)
Human HeLa
NM_003380
1145-1167
aataccaagacctgctcaatg
Human HeLa
NM_003380
863-885
aagaatggtacaaatccaagt
Human HeLa
NM_003380
1037-1059
aaatggaagagaactttgccg
Human HeLa
L18880
2923-2945
aagcggattagaaccaatctc
Mouse 3T3
X99063
1357-1379
aaagcctaccacccacagtgc
Mouse 3T3
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