• RNAi does not efficiently inhibit all genes, an RNAi-based screen will miss some relevant genes (Fraser et al., 2000)
• RNAi may be ineffective against the targeted gene
• RNAi may not accurately phenocopy the null phenotype of all genes and may result in either partial or no loss of function.
•RNAi cannot detect subtle or conditional phenotypes (Fraser et al., 2000)
• Genes encoding neural functions are particularly resistant to RNAi (Fraser et al., 2000)
Advantages of RNAi
•easy to introduce to organism - by feeding or direct injection
•possible to make a library of dsRNA-expressing bacteria that can be used for high-througput genome-wide RNAi screens at very low cost
•vs. the knockout technique - RNAi is faster and less labour intensive. Genes that are lethal when knocked out in embryos can be analyzed with RNAi in cell culture
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