The choice of the right controls makes the whole difference between a good and a bad experiment.
1 Always test the sense and antisense single strands in separate experiments.
2 Try to use a scramble siRNA duplex. This should have the same nucleotide
composition as your siRNA but lack significant sequence homology to any other gene
(including yours).
3 If possible, knock-down your gene with two independent siRNA duplexes to control
the specificity of the silencing process.
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