| 10) Incubate the slides with RNase A (50 µg/ml) for 1 h at room temperature.
11) Blot the slides, dry the area around the sample and add propidium iodide (0.5 µg/ml) for 1 h at room temperature.
12) Wash the slides 4 times in PBS/PVP (2 minutes each).
13) Dry the area around the sample, add 16 µl of mounting medium (w/ antifade) to the slide and cover the sample with coverslip.
14) Determine the number of fluorescent nuclei. Red fluorescence indicates nonapoptotic cells and green/yellow (PI) or (Hoescht)fluorescence indicates apoptotic cells.
Confocal image of a bovine embryo heat shocked at 41 C for 9 h
and subjected to the TUNEL procedure using PI.
Epifluorescent image of a bovine embryo heat shocked at 41 C for 9 h
and subjected to the TUNEL procedure using Hoescht 33342.
上一篇：TUNEL Assay Protocol [Rosen Lab, Baylor College of Medicine] 下一篇：In Situ Apoptosis Detection by TUNEL labeling (Frozen Section) [Wilcox Lab ,Emor
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