胚胎干细胞培养技术大全-试验方案

MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE

Routine Culturing of ES Cells

ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS

MITOMYCIN C TREATMENT OF PMEFs

GAMMA IRRADIATION OF PMEF'S

PMEF FEEDER LAYER CONCENTRATION

ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES

KARYOTYPING ES CELLS

VASECTOMY OF MALE MICE

Normaski Optics

MEDIA FOR EMBRYO CULTURE AND MANIPULATION

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PREPARATION OF MICROINJECTION PIPETTES

EMBRYO MANIPULATION PIPETTES (FLUSHERS ETC)

COLLECTION OF MORULAE and Earlier

Aggregation of ES cells and eight cell stage embryos

BLASTOCYST TRANSFER

Sending sera to testing for mouse pathogens.

SUPEROVULATION OF MICE

FLUSHING OUT OF BLASTOCYSTS

Alizarin Red S (bone) and alcian blue (cartilagestaining of cleared skeletons.

Routine Splitting and freezing of cells

SOFT AGAR ASSAY FOR COLONY FORMATION

VIRUS INFECTION OF FIBROBLASTS

DNA TRANSFECTION OF EUKARYOTIC CELLS USING CALCIUM PHOSPHATE

LIPOFECTAMINE FOR TRANSFORMATION OF EUKARYOTIC CELLS

ELECTROPORATION OF COS-7 CELLS

Growth and transfection of PC12 cells.

CELL CULTURE FROM WHOLE MICE EMBRYO (DAY 10 - 11 PC)

PCR GENOTYPING FROM the EMBRYO YOKE SACK

b-gal staining of eukaryotic cells in vitro

Comments on db-cAMP and forskolin

Comments on Ro 31.8220

IMMUNOFLUORESENCE ON CHAMBER SLIDES

PHOTOMICROGRAPHY

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