L-selectin-PNAd Interactions under Flow Conditions. Flow Chamber Assay
Springer Lab,The CBR Institute for Biomedical Research, Inc. Department of Pathology Harvard Medical School
The main purpose of the flow chamber assay is to visualize and measure interactions between flowing cells expressing a given adhesion molecule on their surface, and their receptor, either directly coated on the flow chamber lower wall or expressed on a cell monolayer.
The present protocol will focus on the interactions between L-selectin expressed on neutrophils and PNAd coated onto the plastic surface.
http://cbr.med.harvard.edu/investigators/springer/lab/protocols/azucena_flow_chamber.html
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