GFP作为指示蛋白，均可以考虑采用含有膜定位信号的GFP，这样在研究转基因细胞时，凡是涉及细胞固定的方法，如：流式细胞仪检测细胞周期、流式细胞仪检测细胞内蛋白表达、免疫组化检测细胞内蛋白表达均可较好的防治GFP的泄漏，从而可以有效的研究目的基因阳性的细胞。
4 参考文献
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