Recommended working medium, trypsin volume and cell inoculation density

Type of Vessels

Culture Area (cm²)

Volume (ml)

Working Medium (ml)

Inoculation Density

Volume of Trypsin (ml)

Nunclon Flask

24

50

7

4.8x10⁵

0.5-0.8

Corning Flask

25

70

7

5x10⁵

0.5-0.8

Falcon Flask

75

250

25

1.5x10⁶

1.0

Nunclon Flask

83

260

30

1.6x10⁶

1.2

Nunclon Flask

175

800

68

3.5x10⁶

2.0

Falcon Dish 35x10mm

9.6

2.5

1.6x10⁵

0.2-0.3

Falcon Dish 65x15mm

21

6.0

4.2x10⁵

0.5-0.6

Falcon Dish 100x20mm

58

16.0

1.1x10⁶

1.0

96 well Plate

0.32

0.2

6x10³

0.01-0.02

24 well Plate

1.88

1.5

3.7x10⁴

0.08-0.1

12 well Plate

3.83

2.0

7.6x10⁴

0.1-0.2

6 well Plate

9.4

3.0

1.8x10⁵

0.2-0.3