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as a custom formulation in 4 ml aliquots (cat. no. 15710-SP).poly-l-lysine solution (Sigma P8920) for 2 minutes. Allow the slides to dry.
Incubate positive and negative control embryos with RNA free DNase (50 U/ml) at 37 (place embryos in box covered with aluminum foil).embryos only - Incubate positive control embryos as described in Step 9. Incubate negative controls in the absence of the enzyme terminal transferase (bottle 1), only with label solution from bottle 2.in the dark.at room temperature in the dark.
for Performing TUNEL Reaction for Embryos Affixed to Microscope Slides
and subjected to the TUNEL procedure using Hoescht 33342.
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