Methods in Molecular Biology VOLUME 180
Transgenesis Techniques Principles and Protocols SECOND EDITION
转基因技术原理与方法 第二版
Edited by Alan R. Clarke
2002 Humana Press Inc.
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内容：
Contributors ......................................................................................... ix
PART I. TOPICAL REVIEWS IN TRANSGENESIS
1 Biomedical and Agricultural Applications
of Animal Transgenesis
Jim McWhir ................................................................................... 3
PART II. TRANSGENESIS IN INVERTEBRATE AND LOWER VERTEBRATE SPECIES
2 Gene Transfer in Drosophila
Mark J. O'Connor and William Chia ........................................ 27
PART III. TRANSGENESIS IN THE MOUSE: OOCYTE INJECTION
3 Oocyte Injection in the Mouse
Gary A. J. Brown and Timothy J. Corbin ............................... 39
PART IV. ALTERNATIVE ROUTES TO THE GERMLINE
4 Adenoviral Infection
Tohru Tsukui and Yutaka Toyoda ........................................... 73
5 Retroviral Infection
Richard A. Bowen ...................................................................... 83
PART V. TRANSGENESIS IN THE MOUSE: THE ES CELL ROUTE
6 In Vitro Isolation of Murine Embryonic Stem Cells
David Wells ................................................................................. 93
7 Production of Chimeras Derived
from Murine Embryonic Stem Cells
David Wells ............................................................................... 127
8 Gene Targeting Strategies
David W. Melton ....................................................................... 151
9 Cre/loxP Recombination System and Gene Targeting
Ralf Kühn and Raul M. Torres................................................ 175
PART VI. CRYOPRESERVATION OF MOUSE LINES
10 Cryopreservation of Transgenic Mouse Lines
Jillian M. Shaw and Naomi Nakagata .................................... 207
11 Ovarian Tissue Transplantation and Cryopreservation:
Application to Maintenance and Recovery
of Transgenic and Inbred Mouse Lines
Jillian M. Shaw and A. O. Trounson ...................................... 229
PART VII. TRANSGENESIS IN THE RAT
12 Transgenesis in the Rat
Linda J. Mullins, Gillian Brooker, and John J. Mullins ...... 255
PART VIII. TRANSGENESIS IN DOMESTIC SPECIES
13 Generation of Transgenic Livestock by Pronuclear Injection
A. John Clark............................................................................ 273
14 Transgenic Sheep from Cultured Cells
Keith H. S. Campbell ............................................................... 289
PART IX. CHARACTERIZATION AND ANALYSIS OF TRANSGENIC STRAINS
15 Analysis of Transgenic Mice
Stefan Selbert and Dominic Rannie ...................................... 305
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