| Glycoprotein Analysis糖蛋白分析手册[Sigma公司] | 点击： 作者：51protocol收集 来源： 时间： 2007-03-19 本站论坛
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|  | Glycoprotein Analysis Mannual 1st Edition糖蛋白分析手册第一版
Sigma公司
Authors:Robert Gates ，Elner Rathbone， Lisa Masterson， Ian Wright， Asgar Electricwala
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Overview
Key to Monosaccharide Symbols,
Abbreviations and Projections . . . . . . . . . . . . 02
Classification and Structure of
Glycan Components
N-Linked Glycans . . . . . . . . . . . . . . . . . . . 05
O-Linked Glycans . . . . . . . . . . . . . . . . . . . 09
GPI Anchored Proteins . . . . . . . . . . . . . . . 12
Glycoprotein Purification
m-Aminophenylboronate Matrices . . . . . . . . 13
Lectin Matrices . . . . . . . . . . . . . . . . . . . . . . . . 13
Glycoprotein Detection
Colorimetric Detection on PAGE and Blots . . . 15
Fluorescent Detection on PAGE . . . . . . . . . . . 16
Biotin Labeling on Blots . . . . . . . . . . . . . . . . . 17
Glycoprotein Standards . . . . . . . . . . . . . . . . . 18
Chemical Deglycosylation Strategies
Hydrazinolysis. . . . . . . . . . . . . . . . . . . . . . . . . 22
Alkaline b-Elimination . . . . . . . . . . . . . . . . . . 23
Trifluoromethanesulfonic Acid. . . . . . . . . . . . 23
Enzymatic Deglycosylation Strategies
N-Linked Glycan Enzymatic Hydrolysis . . . . . . 24
Native and Sequential Hydrolysis . . . . . . . . . . 25
O-Linked Glycan Enzymatic Hydrolysis . . . . . . 29
Endoglycosidases . . . . . . . . . . . . . . . . . . . . . . 32
Glycan Labeling and Analysis
Glycan Labeling . . . . . . . . . . . . . . . . . . . . . . . 36
HPLC Analysis . . . . . . . . . . . . . . . . . . . . . . . . . 37
Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . 39
NMR Analysis . . . . . . . . . . . . . . . . . . . . . . . . . 45
Electrophoresis . . . . . . . . . . . . . . . . . . . . . . . 45
Glycan and Carbohydrate Standards
N-Linked High Mannose. . . . . . . . . . . . . . . . . 47
N-Linked Hybrid . . . . . . . . . . . . . . . . . . . . . . . 49
N-Linked Complex . . . . . . . . . . . . . . . . . . . . . 49
N-Linked Fragments . . . . . . . . . . . . . . . . . . . . 55
O-Linked Neutral Glycans. . . . . . . . . . . . . . . . 57
O-Linked Sialylated Glycans . . . . . . . . . . . . . . 60
Blood Group Antigens . . . . . . . . . . . . . . . . . . 63
Lewis and Cell Adhesion Glycans . . . . . . . . . . 65
Gal a(1,3) Gal Antigens . . . . . . . . . . . . . . . . . 67
Monosaccharides . . . . . . . . . . . . . . . . . . . . . . 67
Glycobiology Product Directory
Additional Information Sources:
Enzyme Explorer. . . . . . . . . . . . . . . . . . . . . . . 68
Complete List of Glycolytic Enzymes . . . . . . . 69
GPI Anchor Enzymes. . . . . . . . . . . . . . . . . . . . 74
Glycosyltransferases . . . . . . . . . . . . . . . . . . . . 74
Inhibitors of Carbohydrate Metabolism . . . . . . 75
Glycolytic Enzyme Substrates . . . . . . . . . . . . . 76
Neoglycoproteins . . . . . . . . . . . . . . . . . . . . . . 79
Lectins. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
上一篇：Two-Hybrid Systems Methods and Protocols酵母双杂交系统实验方法[HUMANA PRESS] 下一篇：Membrane Protein Protocols膜蛋白表达、纯化、鉴定方法[HUMANA PRESS] | | |
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