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Handbook of ELISPOT ELISPOT实验方法手册，经典的ELISPOT教程[2005 Humana Press]

点击： 作者：51protocol收集 来源： 时间： 2007-03-22 　本站论坛

Handbook of ELISPOT Methods and Protocols ELISPOT实验方法手册 Edited by Alexander E. Kalyuzhny 2005 Humana Press Inc. PDF格式，29M，如需下载此书，请在论坛留言。 Contributors .................................................................................................i.r.. .
The ELISPOT Assay: A Personal Retrospective
Jonathon D. Sedgwick ........................................................................... 3
Chemistry and Biology of the ELISPOT Assay
Alexander E. Kalyuzhny ...................................................................... 15
Membranes and Membrane Plates Used in ELISPOT Alan J. Weiss. ...................................................................................... 33
Standardization and Validation Issues of the ELISPOT Assay
Sylvia Janetzki, Josephine H. Cox, Neal Oden,
and Guido Ferrari ........................................................................... 51
A Cell-Detachment Solution Can Reduce Background Staining
in the ELISPOT Assay
Angela Grant, Sarah Palzer, Chris Hartnett, Tanya Bailey,
Monica Tsang, and Alexander E. Kalyuzhny .................................. 87
Isolation of Subsets of Immune Cells
Carrie E. Peters, Steven M. Woodside, and Allen C. Eaves ................ 95
Image Analysis and Data Management of ELISPOT
Assay Results
Paul Viktor Lehmann ........................................................................ 117
High Resolution as a Key Feature to Perform Accurate ELISPOT
Measurements Using Zeiss KS ELISPOT Readers Wolf Malkusch .................................................................................. 133
Improving the Sensitivity of the ELISPOT Analyses of Antigen-Specific
Cellular Immune Responses in Rhesus Macaques
K. Jagannadha Sastry, Pramod N. Nehete, and Bharti Nehete ......... 153
1 0 Feline Cytokine ELISPOT: Issues in Assay Development
Sushila K. Nordone, Rosemary Stevens, Alora S. LaVoy,
and Gregg A. Dean ....................................................................... 167 11Measuring T-Cell Function in Animal Models of Tuberculosis
by ELISPOT
Vanja lazarevic, Santosh Pawar, and JoAnne Flynn ........................
12 Interferon-y ELISPOT Assay for the Quantitative Measurement
of Antigen-Specific Murine CD8' T-Cells Geoffrey A. Cole ............................................................................... PART Ill. ELISPOT ASSAYS IN HUMAN STUDIES
13 Detection of Measles Virus-Specific Interferon-y-Secreting
T-Cells by ELISPOT Jenna E. Ryan, lnna G. Ovsyannikova, and Gregory A. Poland ....... 207
Epitope Mapping in Multiple Sclerosis Using the ELISPOT Assay
Clara M. Pelfrey and loana R. Moldovan ......................................... 219
Use of Interferon-y ELISPOT in Monitoring Immune Responses
in Humans
Mark Matijevic and Robert G. Urban ............................................... 237
ELISPOT Determination of Interferon-y T-Cell Frequencies
in Patients With Autoimmune Sensorineural Hearing Loss
C. Arturo Solares and Vincent K. Tuohy .......................................... 253
PART IV. MULTIPLEX AND MODIFIED ELI SPOT ASSAY FORMATS
17 Dual-Color ELISPOT Assay for Analyzing Cytokine Balance
Yoshihiro Okamoto and Mikio Nishida ............................................ 263
Simultaneous Detection of Multiple Cytokines in ELISPOT Assays
Sarah Palzer, Tanya Bailey, Chris Harfneft, Angela Grant,
Monica Tsang, and Alexander E. Kalyuzhny ................................ 273
1 9 Fluorospot Assay: Methodological Analysis
Agnes Gazagne, Wolf Malkusch, Benoit Vingert,
Wolf H. Fridman, and Eric Tartour ............................................. 289
20 A Gel-Based Dual Antibody Capture and Detection Method
for Assaying of Extracellular Cytokine Secretion: EliCell
Lisa A. Spencer, Rossana C. N. Melo, Sandra A. C. Perez,
and Peter F. Weller ....................................................................... 297
Index .........................................................................................................3..1. 5

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