Protocol for Sending Strains菌种运输方法

Dr. DE Koshland, Carnegie Institution of Washington

http://www.ciwemb.edu/labs/koshland/Protocols/YEAST/sendstrains.html

1. Sterile pieces of foil (about 2.5 inches square). (Sterilize and store in a glass petri dish)
   
2. 1/4" sterile disks (from Difco, #1599-35, concentration disks 1/4", sterile blanks)
   
3. Remove a disk from the bottle using a sterile toothpick. place on a piece of sterile foil
   
4. Wet disk with a drop of YEPD (yeast) or LB (bacteria).
   
5. Pick a single colony from a plate with a toothpick and transfer to sterile disk
   
6. Fold ends of foil to wrap disk, fold ends several times to prevent leaking
   
7. Wrap with a piece of white tape and label with strain number
   
   These can be taped to a sheet of paper with the genotype and mailed in a regular envelope