Sequence of HAT tag (3xHA):
TR in upper case. loxR in bold.
GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGgc ggccattgaa ggtagaagag aaaatttgta cttccaaaga aagaaggccg ctatcgcttc ggataactcc tgctatacga agttatgggc ggccgtttac ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta tgcaggatcc tatccatatg acgttccaga ttacgctccg gccgcCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCC
GenBank accession
Antibiotics used:
| Tetracycline, Tet (Sigma T3383) |
12 mg/ ml in 50% ethanol. Use at 3 ug/ml (Tet3) |
| Kanamycin, Kan (Sigma K800) |
10 mg/ ml in water. Use at 40 ug/ml (Kan40) |
| Ampicillin, Amp (Sigma A9518) |
50 mg/ml in water. Use at 50 ug/ml (Amp50) |
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