Cell Lysates For Western Blotting

Reagents / Solutions

Protocol

1. Spin down 10⁶ cells and wash, if required, in phosphate buffered saline.
2. Resuspend cells in 100µl warmed lysis buffer, vortex then boil for 5 - 10 minutes to break up DNA.
3. Cool (not on ice - the SDS will precipitate) and centrifuge to collect droplets.
4. Vortex briefly to mix.
5. The samples may now be analysed by , or
6. Store samples at -20°C until required.