David R. Caprette, Rice University
The principle of the biuret assay is similar to that of the Lowry, however it involves a single incubation of 20 min. There are very few interfering agents (ammonium salts being one such agent), and Layne (1957) reported fewer deviations than with the Lowry or ultraviolet absorption methods. However, the biuret assay consumes much more material. The biuret is a good general protein assay for batches of material for which yield is not a problem. The Bradford assay is faster and more sensitive
http://www.ruf.rice.edu/~bioslabs/methods/protein/biuret.html 上一篇：Measurement of Myosin Regulatory Light Chain (RLC) Phosphorylation 下一篇：GBuilder 1.23
