the Tansey Lab at ColdSpringHarbor Laboratory
This assay is performed to directly measure the stability of a specific protein in yeast. Following pulselabeling of yeast cells, recover your target protein by denaturing immunoprecipitation, as described in the ‘Ultimate Yeast Denaturing IP’ protocol. If you have difficulty detecting your labeled protein, try increasing the amount of labeled methionine used. If you are using temperature-sensitive yeast, shift to the restrictive temperature during the last 30 minutes of the starvation period (B.2).
http://tanseylab.cshl.edu/pdf/PC_Yeast.pdf
