Fluorescein labeling of proteins
David Chambers,Salk institute
This method uses carboxyfluorescein succinimidyl ester (CFSE) rather than fluorescein isothiocyanate, resulting in more reliable labeling. Succinimidyl esters are excellent reagents for amine modification since the amide products formed are very stable. CFSE has high reactivity with aliphatic amines, low reactivity with aromatic amines, including tyrosine.
http://pingu.salk.edu/flow/protocols/flabel.html 上一篇：Pyxis 2.0 下一篇：Labeling Gizzard Myosin with Tetramethyorhodamine Iodoacetamide (MHC and 17kD ML
