| Prowl Lab,Rockefeller University
http://prowl.rockefeller.edu/recipes/sproteas/chymot.htm
Specificity
Cleaves on the C-terminal side of F, Y, W and L. Some cleavage reported C-terminal to M, I, S, T, V H, G and A.
pH optimum
7.5 - 8.5
Stability
Functions in the presence of 2 M guandidine hydrochloride and 0.1% SDS. Store lyophilized or in 1 mM HCl and 2 mM calcium chloride.
Recipe
Dissolve protein substrate at up to 10 g/l in 100 mM ammonium bicarbonate. Add enzyme solution to give a 50:1 ratio of substrate to enzyme. Incubate for at least 4 hours at 37 C.
Spackman, D.H., Stein, W.H. and Moore, S. The disulfide bonds of ribonuclease. J. Biol. Chem. 235(1960)648-659.
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