Chazin Lab, Center for Structural Biology, Vanderbilt University
This protocol describes the general procedure for setting up a limited proteolysis reaction. An experiment like this is useful for determining the sizes, timed order of appearance, and relative amounts of fragments produced from the protein of interest by digestion with a protease. From the results, you may be able to infer the presence of stable subdomains. ……
http://structbio.vanderbilt.edu/chazin/wisdom/labpro/proteolysis.html
