Hahn Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute
WCE Extracts are prepared from strain SHY282 (TFC4-Flag-EE (HpaI)). TFIIIC complex is first purified by flag affinity: TFC4 contains a single copy of the flag epitope. The flag purified TFIIIC is then further purified by anion exchange on a Source Q column.
