3. 检测器中充满缓冲液，看记录笔的位置，最好接近～10％刻度的地方，这样防止出现吸光度值低于缓冲液无法记录的情况；如果笔位太高，应增大检测器信号输出，重新按步骤2 调节；反之降低信号输出；有的记录仪也可调节输入信号的范围，这样子就更方便了。满刻度的调节要视具体应用，不一定是1mg/ml 的蛋白浓度。
9、about dimension of column:（davidzrock） W/~- A8
"The choice of inner diameter may be based on the desired sample load or on considerations of the extra XS<Sp,v]
dispersion |~F}tjQs
effects from the wall region,which extends 20 particle diameters away from the wall. ... The length of the column p$5*Pp
is primarily chosen according to the resolution that is required." KHCa23ypw
"The dimensions may be chosen according to the application at hand, but most laboratory columns have a 4- to $W#_YMax
16-mm inner diameter(i.d.) and a length of 25 to 70 cm." 1?%n&jg *
-----From ‘Protein Purification principles,High Resolution Methods,and Applications’ 2ed edited by J.C. Janson (hx8rf6j>}
and L.Ryden P(qV70GWJ1
"The resolution of two separated zones in gel filtration increases as the sequare root of column length. Long LQDgyp*9
columns should,therefore,be used to obtain the best resolution in analytical fractionation. Bed heights of greater P#"m9A
than 1m are seldom required. If a very long bed is judged to be necessary ,the effective bed height can often be ZqH>U}Jd
increased simply by recycling or by using columns coupled in series."
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