1M NaOH 处理1hr，可有效地减少微生物污染。具体使用哪种消毒剂以及消毒方法，请参阅凝胶及空柱使用说明书，避免损害凝胶柱。如你没有，我可帮你查。
3.柱子长期不用时，为防止微生物生长，建议在位保存。如用10mM NaOH或20％乙醇将柱内的液体全部置换出来，存放在建议温度下（不一定是低温）。使用前再进行冲洗和平衡。也可将凝胶取出，保存于上述溶液中。
4.如有微生物生长，应进行灭菌处理，如1M NaOH 或取出凝胶进行高温灭菌，具体方法亦需事先参阅说明书。
From Amersham t`U,T)3
Antimicrobial agents for chromatography r*oS{O 7
Aqueous buffer solutions will often support growth of micro-organisms commonly found in laboratories. Since it is difficult to clean columns that have become heavily contaminated with microorganisms, precautions should be taken to avoid growth in the column or in buffer vessels. Always use fresh buffer solutions and cover the buffer vessels to keep out dust, spores and other particles. If a column will not be used for more than a couple of days, it should be equilibrated with the bacteriostatic agent described in the manual supplied with the separation medium or prepacked column. We recommend ethanol or sodium hydroxide for general use. These agents are effective, inexpensive and do not cause disposal problems. Ethanol 20% Many chromatography media from Amersham Biosciences are supplied as a suspension containing 20% ethanol. Ethanol, 20%, can also be used as an alternative to NaOH for storing chromatography media under bacteriostatic /cYes9O.x
tS%e>c@$B
conditions. It should not be used for columns packed with Sephadex G-types and other media based on Sephadex since the bed will shrink and spoil the packing. Sodium hydroxide Sodium hydroxide, 0.01 M, is an effective bacteriostatic agent. At higher concentrations (0.5-1.0 M) it is an g5 }j^h
effective sanitizing agent for contaminated columns. For the most frequent contaminants in chromatographic systems, such as gram-negative bacteria, a good bactericidal effect is reached even at concentrations as low as pt9<7c]2i(
上一篇：Pyrosequencing 原理概述 下一篇：重组Escherichia coli的高密度培养
共20页: 上一页 [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] 18 [19] [20] 下一页
