SDS-PAGE分离，KCL显带，割胶，而后电渗分离，测OD 值极低，请问哪位有这方面的经验或其它方法，望赐教。解决：多挖点胶，收集起来，放在1.5 离心管里，用PBS捣烂，4度 过夜。然后离心，收集上清即可。最后，把上清冻成干粉。
2、问题：蛋白质提纯的方法：
SDS-PAGE and N-terminal amino acid sequence analysis.（dahuaidang）SDS polyacrylamide gel electrophoresis (SDS-PAGE) was performed in 12% acrylamide and 0.1% SDS with adiscontinuous Tris-glycine buffer system. Purified enzyme was subjected to SDS-PAGE and electroblotted toImmobilo-PSQ 0.45μm polyvinylidene fluoride membrane (Millipore). After the membrane was stained with ~?$ki =7
Ponceau S (Sigma), the enzyme protein was cut out and washed with methanol. The membrane strip holding the protein was subjected to automatic Edman degradation for sequencing of the N-terminal aminol acids using an Applied Biosystems 477A protein sequencer (Parkin Elmer).
3、问题：蛋白质纯化，装柱，过柱应注意事项有哪些?
现在要进行蛋白质纯化，有关装柱和过柱的注意事项有哪些呀？还有，需要测序的蛋白质样品，大概需要多少，样品要求有哪些。柱子sephadex-200,和
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