|
6．Tris-TBS(PBS)
试剂：Triton X-100 10ml(1%)或3ml(0.3%)
0.5mol/L Tris缓冲液（pH7.6） 1000ml(50ml)或(0.2mol/L的PB)
NaCl 8.5～9g
重蒸水 至1000ml
配制方法：先以重蒸水少许溶解NaCl后，加入Triton X-100及Tris缓冲液或（PB），最后加重蒸水至1000ml，充分摇匀。
该液常用浓度为1%及0.3%，前者主要用于漂洗标本，后者主要用于稀释血清。
7．0.1mol/L（pH7.4）二甲胂酸缓冲液
试剂：0.2mol/L二甲胂酸钠 500ml
0.1N HCl 28ml
蒸馏水 至1000ml
配制方法：先称取二甲胂酸钠（MW：214）42.8g,加蒸馏水至1000ml，使0.2mol/L的二甲胂酸钠溶液；再取HCl 1.7ml加蒸馏水至1000ml ,配成0.1N，最后取0.2mol/L二甲胂酸钠溶液500ml及0.1n HCl 28ml混合，加蒸馏水至1000ml，即为0.1mol/L的二甲胂酸钠缓冲液。
8．几种常用的不同pH值缓冲液的配制表
（1）0.2mol/L磷酸盐缓冲液（pH5.7～8.0）
|
pH
|
0.2mol/L NaH2PO4(ml)
|
0.2mol/L Na2HPO4(ml)
|
|
5.7
|
93.5
|
6.5
|
|
5.8
|
92.0
|
8.0
|
|
5.9
|
90.0
|
10.0
|
|
6.0
|
87.7
|
12.3
|
|
6.1
|
85.0
|
15.0
|
|
6.2
|
81.5
|
18.5
|
|
6.3
|
77.5
|
22.5
|
|
6.4
|
73.5
|
26.5
|
|
6.5
|
68.5
|
31.5
|
|
6.6
|
62.5
|
37.5
|
|
6.7
|
56.5
|
43.5
|
|
6.8
|
51.0
|
49.0
|
|
6.9
|
45.0
|
55.0
|
|
7.0
|
39.0
|
61.0
|
|
7.1
|
33.0
|
67.0
|
|
7.2
|
28.0
|
72.0
|
|
7.3
|
23.0
|
67.0
|
|
7.4
|
19.0
|
81.0
|
|
7.5
|
16.0
|
84.0
|
|
7.6
|
13.0
|
87.0
|
|
7.7
|
10.5
|
89.5
|
|
7.8
|
8.5
|
91.5
|
|
7.9
|
7.0
|
93.0
|
|
8.0
|
5.3
|
94.7
|
(2)0.05mol/L Tris-HCl缓冲液（pH7.19～9.10）
|
pH
|
0.2mol/L Tris(ml)
|
0.2mol/L HCl(ml)
|
H2O
|
|
7.19
|
10.0
|
18.0
|
12.0
|
|
7.36
|
10.0
|
17.0
|
13.0
|
|
7.54
|
10.0
|
16.0
|
14.0
|
|
7.66
|
10.0
|
14.0
|
15.0
|
|
7.77
|
10.0
|
14.0
|
16.0
|
|
7.87
|
10.0
|
13.0
|
17.0
|
|
7.96
|
10.0
|
12.0
|
18.0
|
|
8.05
|
10.0
|
11.0
|
19.0
|
|
8.14
|
10.0
|
10.0
|
20.0
|
|
8.23
|
10.0
|
9.0
|
21.0
|
|
8.32
|
10.0
|
8.0
|
22.0
|
|
8.41
|
10.0
|
7.0
|
23.0
|
|
8.51
|
10.0
|
6.0
|
24.0
|
|
8.62
|
10.0
|
5.0
|
25.0
|
|
8.74
|
10.0
|
4.0
|
26.0
|
|
8.92
|
10.0
|
3.0
|
27.0
|
|
9.10
|
10.0
|
2.0
|
28.0
|
(3)0.2mol/L醋酸盐缓冲液（pH3.6～5.6）
|
pH
|
0.2mol/L醋酸（ml）
|
0.2mol/L醋酸钠（ml）
|
|
3.6
|
46.3
|
3.7
|
|
3.8
|
44.0
|
6.0
|
|
4.0
|
41.0
|
9.0
|
|
4.2
|
36.8
|
13.2
|
|
4.4
|
30.5
|
19.5
|
|
4.6
|
25.5
|
24.5
|
|
4.8
|
20.0
|
30.0
|
|
5.0
|
14.8
|
35.2
|
|
5.2
|
10.5
|
39.5
|
|
5.4
|
8.8
|
41.2
|
|
5.6
|
4.8
|
45.2
|
(4)0.1mol/L二甲胂酸钠缓冲液（pH5.0～7.4）
|
pH
|
0.2mol/L二甲胂酸钠（ml）
|
0.2N HCl(ml)
|
蒸馏水
|
|
5.0
|
25
|
23.5
|
51.5
|
|
5.2
|
25
|
22.5
|
52.5
|
|
5.4
|
25
|
21.5
|
53.5
|
|
5.6
|
25
|
19.6
|
55.5
|
|
5.8
|
25
|
17.4
|
57.5
|
|
6.0
|
25
|
14.8
|
60.3
|
|
6.2
|
25
|
11.9
|
63.1
|
|
6.4
|
25
|
9.2
|
65.8
|
|
6.6
|
25
|
6.7
|
68.3
|
|
6.8
|
25
|
4.7
|
70.3
|
|
7.0
|
25
|
3.2
|
71.8
|
|
7.2
|
25
|
2.1
|
72.9
|
|
7.4
|
25
|
1.4
|
73.6
|
注：HCI可由NCO3代替，配制成二甲胂酸钠-HNO3缓冲液。
（5）0.2mol/L碳酸盐缓冲液（pH9.2～10.7）
|
pH
|
0.2mol/L Na2CO3(ml)
|
0.2mol/L NaHCO3(ml)
|
|
9.2
|
4.0
|
46.0
|
|
9.3
|
7.5
|
42.5
|
|
9.4
|
9.5
|
40.5
|
|
9.5
|
13.0
|
37.0
|
|
9.6
|
16.0
|
34.0
|
|
9.7
|
19.5 | | 
