In-gel Tryptic Digest for Protein ID by Mass Spectrometry
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  • In-gel Tryptic Digest for Protein ID by Mass Spectrometry

  • 点击:    作者:51protocol收集   来源: 日期:2007-03-22    本站论坛
Mitshison Lab, Department of Systems Biology, Harvard Medical School This protocol is based on Shevchenko A, Wilm M, Vorm O, & Mann M. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal Chem 1996, 68:850-8. I have used it with success on both Coomassie and silver-stained gel bands. …… http://mitchison.med.harvard.edu/protocols/MSTrypticDigest.htm

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