• Antibiotic Concentration in Media

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Antibiotic Concentration in Media

Antibiotics should be added to lower than 60 C broth, or filter sterilized: See note. "C' refers to chromosomal resistance: "P" plasmid based resistance, "FS" filter sterilization required. Temperature is required for storage.
Concentration (µg/ml)

Antibiotics for Pseudomonas, E. Coli, Agrobacterium, etc...
Ampicillin 1000 (C/P) 50 (P) Stock: 25 mg.ml Na salt:FS: Store at -20 C, carbenicillin is more stable than Amp.
Carbenicillin 1000 (C/P) 50 (P) 100 mg/ml: FS: -20 C
Chlroramphenicol 500 (C) 30 (C/P) 170 µg/ml for plasmid amplification 34 mg/ml in EtOH: -20 C
D-cycloserine 200 (C) Add dry after autoclaving
Erythromycin 200 (C) 25 mg/ml EtOH: -20 C
Gentamycin 10 (C/P) Add dry after autoclaving
Geneticin 200 for fungi Same as kanamycin in mode of action and preparation
Hygromycin 250 for fungi 25 mg/ml: FS: -20 C
Kanamycin 200 (P) 50 (P) 25 mg/ml: FS: -20 C
Kasugamycin 200 (C) 20 mg/ml: FS: -20 C
Mercuric chloride 40(C/P) 10 µg/ml if in minimal media, 40 mg/ml in EtOH: -20 C
Nalidixic acid 1000 (C) 20 (C) 100 mg/ml in H2O, add 6 N NaOH until dissolved: 4 C
Neomycin 200 (P) 50 (P) 25 mg/ml: FS: -20 C
Rifampicin 200 (C) 200 mg/ml make fresh in Methanol
Spectinomycin 200 (C/P) 25 (P) 20 mg/ml: FS: -20 C
Streptomycin 200 (C/P) 25 (P) 20 mg/ml: FS: -20 C
Sulfamethoxazole 500(C) Add dry after autoclaving
Triomethoprim 500 (C) Add dry after autoclaving
Tetracycline 20(C)50(P) 15 (P) 12.5 mg/ml 50 % EtOH: -20 C