| 试剂配方:DNA操作试剂 | | 点击： 作者： 来源： 时间： 1970-01-01 本站论坛 |
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0.1% SDS 10% SDS 40 ml
dH2O to 4000 ml
Washing off Probe for Re-hybridization of Blots (II)
Washing time: 3 min
Final conc. Stock Vol.
0.1 N NaOH 10 N NaOH 40 ml
0.2% SDS 10% SDS 80 ml
dH2O to 4000 ml
Washing off Probe for Re-hybridization of Blots(Ⅲ)
Washing time: 20 min
Final conc. Stock Vol.
0.2 M Tris. (pH 7.5) 1 M Tris. (pH 7.5) 800 ml
0.1×SSC 20×SSC 20 ml
0.2% SDS 10% SDS 80ml
dH2O to 4000ml
Blue Juice
Final conc. Stock Vol. Vol.
70% Glycerol 100% 35 ml 70 ml
0.5×TBE 5× 5 ml 10 ml
0.2% SDS 10% 1 ml 2 ml
20 mM EDTA 0.5 M 2 ml 4 ml
5 mg/ml Bromphenol Blue 0.25 g 0.5 g
5 mg/ml Xylene cyanol 0.25 g 0.5 g
dH2O to 50 ml 100 ml
EB (10 mg/ml)
ehidium bromide 1 g
dH2O to 100 ml
Stir on a magnetic stirrer for several hours. Transfer the solution to
a dark bottle and store at 4℃.
The concentration of work solution: 0.5 μg/μl (50 μl stock solution
In 1000 ml dH2O).
Decontamination of EB
Reduce the concentration of EB <0.5 mg/ml, add 1 volume of
0.5 M KMnO4,mix carefully then add 1 volume of 2.5 N HCl,
mix carefully and allow the solution to stand at room temperature
for several hours. Add 1 volume of 2.5 N NaOH, mix and discard.
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