Phosphate (Sodium) buffer ChartStock solution A
2 M monobasic sodium phosphate, monohydrate (276g/L)
Stock solution B
2 M dibasic sodium phosphate (284 g/L).
Mixing an appropriate volume (ml) of A and B as shown in the table below and diluting to a total volume of 200 ml, a 1 M phosphate buffer of the required pH at room temperature.
A B pH
92.0 8.0 0.8
90.0 10.0 5.9
87.7 12.3 6.0
85.5 15.0 6.1
81.5 19.5 6.2
77.5 22.5 6.3
73.5 26.5 6.4
68.5 31.5 6.5
62.5 37.5 6.6
56.5 43.5 6.7
51.0 49.0 6.8
45.0 55.0 6.9
39.0 61.0 7.0
33.0 67.0 7.1
28.0 72.0 7.2
23.0 77.0 7.3
19.0 81.0 7.4
16.0 84.0 7.5
13.0 87.0 7.6
10.5 89.5 7.7
8.5 91.5 7.8
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