Silane and Poly-L-Lysine Coating of Microscope Slides
HTTP_REFERER: http://www.ccc.nottingham.ac.uk/~mbzspd/MSF.html.
Submission Details: *Jill Fergusson*Department of Biochemistry*University of Nottingham*U.K.*14:05*22/2/96.
Database: Method/Protocol1) Wash slides in Decon solution for 30 minutes.
2) Wash slides in running tap water for 30 minutes.
3) Wash slides in distilled water for 2 x 5 minutes.
4) Wash slides in 95% ethanol for 2 x 5 minutes.
5) Air dry in a dust free environment for 5 minutes.
Poly-L-Lysine Coating
a) Smear 20ul of poly-l-lysine over each slide. b) Air dry in a dust free environment.
Silated Slides
a) To 300mls acetone add 6mls Aminopropyltriethoxysilane solution (Sigma A-3648). b) Dip slides into silane solution for 10 seconds. c) Wash 2 x briefly in distilled water. d) Dry overnight at 37°C in a dust-free environment.
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