OBJECTIVE:
This tests for the presence of electron transport system in Pseudomonas species.
MATERIALS:
- Filter paper
- 1% N,N,N'N' tetramethyl-p-phenylenediamine
METHODS:From Colonies:
- Pre-wet filter paper with oxidase reagent (N,N,N',N'-p-phenylenediamine) and allow to dry.
- Pick a bacterial colony with a sterile toothpick.
- Gently scratch the colony onto the filter paper. A blue color is produced by Pseudomonas species.
From Liquid Culture:
- Spot the filter paper with 10 to 20 ?l of culture.
- Add 10 ?l of freshly made oxidase reagent and look for production of a blue color.
NOTE: If the filter paper is pre-wetted with oxidase reagent, false positive can occur.
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