| Phenol Sulphuric Acid Carbohydrate Assay | | 点击： 作者：51protocol收集 来源： 时间： 2007-03-18 本站论坛 |
|  | REFERENCE: Anal. Chem. 28:(1956) 350-356.
OBJECTIVE:
To detect the presence of sugars. (Widely used method to monitor column eluates for elution of sugar containing compounds such as LPS, 0-antigen, etc.)
MATERIALS:
- Standards:glucose 1 mg/ml stock solution
- use 10, 20, 40, 60, 80 µl and make up each sample with dH2O to a final volume of 100 µl.
- Blank 100 µl water
- Samples:50 µl (of 5 mg/ml LPS solution) and make up to 100 µl.
METHOD:
- Add 50 ul of 80% Phenol solution (80% Phenol by weight)
- Vortex. Add 2.0 ml concentrated Sulphuric Acid in a stream.
- Stand 10 min. at room temperature.
- Read absorbance an 490nm.
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