Preparing Lawn Cells for M13 Cloning (Life Technologies) Lawn cells require the F' episome for M13 infection and may be prepared
Streaking Lambda Phages (Donis Keller Lab) Phage are streaked onto a medium to obtain an independent isolate prior to preparing a new lysate
Phage Propagation (hancock Lab) (Accessible only by Internet Explorer)
Helper Phage Preparation (Vesicle Trafficking)
Picking Single Phage Plaques(Donis Keller Lab) For isolating single bacteriophage lambda plaques to be used for making lysates
(Molecular Genetics Network Lab)
Liquid Phage Lysates (Donis Keller Lab)
Preparation of Phage Lysates (Goldberg Lab) Very nice and detailed protocol including recipes for media preparation
Gel Assay to Determine DNA Content of Phage Lysates (Donis Keller Lab) A crude lysate gel assay can be performed to roughly quantitate the DNA in lysates. This is often a valuable time saving step to determine if the phage yield is sufficient to warrant continuing the procedure
Titering Lambda Phages (Donis Keller Lab) This procedure is used to find the number of viable phage particles in a lysate. The phage lysate is serially diluted and ...
Phage Plate Stock Lysates (Donis Keller Lab) To prepare phage lysates to be used for small or large scale phage DNA preps. This method usually produces lysates with titers of 2-8x10e10 pfu/ml.
Long-Term Lambda Phage Storage (Donis Keller Lab) Store phage in 7% DMS0.
Phage and Cell Storage (Gimila Lab)
Transfer of Phage DNA from Plaques to Nylon Membrane or Nitrocellulose (Donis Keller Lab) To transfer phage DNA to nylon membranes so that a phage library can be screened by hybridization
Assay for Phage Containing the ß- Galactosidase Gene (Donis Keller Lab) This assay is used when working with phage vectors carrying the beta-galactosidase gene (often used for immunological screening). If the cloning event disrupts a normally functional copy of the gene in the vector the resulting plaques would appear clear in the assay. If the phages contain a functional beta-galactosidase gene they will form blue rings around their plaques.
Plaque Lifts (Gimila's Lab)
Identifying Plaques by Neutral Red Staining (LTI)
Phage Titre and Solution Preparation (Gimila's Lab)
Colony/Plaque Blotting (Gimila's Lab)
(Goldberg Lab)
Phage Media Diagram (Goldberg Lab) procedures for phage media preparation
Growth and Purification of 25-100 µg Lambda Clone DNA (Donis Keller Lab)
Lambda Methods Media and Solutions (Donis Keller Lab)
Isolation of Phage Resistant Mutants (Hancock Lab) (Accessible only by Internet Explorer)
