细菌检测

• Gram Staining (+\-) (William H. Heidcamp)
    
• Gram-Staining Procedure (MEDIC, U of Texas)
  Very nice and detailed method description for Gram staining
    
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  To detect the presence of acid-fast mycobacteria in tissue sections
• Smear Preparation (MEDIC, U of Texas)
  The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium.
    
• Alkaline Phosphatase and Phospholipase C Assay (Hancock Lab) (Accessible only by Internet Explorer)
    
• Bacteria Agglutination Assay (Hancock Lab) (Accessible only by Internet Explorer)
  Bacterial agglutination is performed using either P. aeruginosa cells autoclaved at 120^oC for 1 hour, then centrifuged and resuspended in saline or live P. aeruginosa cells grown to mid log phase, then centrifuged and resuspended in saline.
     
• Carbohydrate Assay(Hancock Lab) (Accessible only by Internet Explorer)
  This assay is used to determine the relative amounts of LPS CHO present in a given strain and can be done on one set of samples and then scanned at the usr/localious wavelengths for reasonable data on the 3 types of sugars.
    
• Dintrophenylation Assay for Polymixin B (Hancock Lab) (Accessible only by Internet Explorer)
    
• Endotoxin Assay(Hancock Lab) (Accessible only by Internet Explorer)
  This assay measures the shift in the absorbance maximum of the dye due to binding to endotoxin
    
• Indirect Immunfluoresence (Hancock Lab) (Accessible only by Internet Explorer)
    
• Liposome Swelling Assay (Hancock Lab) (Accessible only by Internet Explorer)
  Also including method for liposome Isolation and  liposome preparation
    
• Preparation of E Coli Protein for SDS-PAGE (Hahn Lab)
  Simplified method for analyzing E coli protein