细菌的核酸抽提
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  • 细菌的核酸抽提

  • 点击:    作者:   来源: 日期:2006-11-09    本站论坛

DNA Extraction

·         (Julie B. Wolf,UMBC)
Phenol/chloroform method

·         DNA Extraction From Bacteria (Triton Method) (NWFSC)

  • Isolation of Chromosomal DNA from Bacteria (Hancock Lab) 
        

·         Rapid Genomic DNA Isolation from P. Aeruginosa (Goldberg Lab)

·         DNA Extraction from Gram-positive Bacteria (NWFSC)
This procedure was originally developed for Listeria monocytogenes but has worked well with other Gram+ bacteria

·         (Newman Lab)

·         (Lazo Lab)
Phenol/chloroform method

·         Genomic DNA Isolation (Jeffrey D. Newman)
Phenol/chloroform method, this procedure does not work well with Gram + cocci

  • (Molecular Genetics Network Lab)
     
  • Isolation of Aspergillus DNA: QUIAGEN Method (Molecular Genetics Network Lab)
      

·         Isolation and Quantification of Genomic DNA from Mycobacterium tuberculosis (The Northeast Regional RFLP Laboratory for DNA Typing)
This protocol describes a "DNA mini-prep" procedure for the isolation of chromosomal DNA from Mycobacterium tuberculosis and a procedure for quantifying the amount isolated

RNA Extraction

·         Isolation Of RNA From Bacteroids and Bacteria (Lazo Lab)

  • (Molecular Genetics Network Lab)
      
  • Extraction of RNA from Aspergillus: - Guanidin-thiocyanate and LiCl method (Molecular Genetics Network Lab)

Others

·         Pulsed-field Gel Purification of E. coli Restriction Fragments (Stanford DNA Sequence & Technology Center)

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