细菌培养-试验方案

Preparing Overnight Bacteria Culture (LaboratoryExperiments.com)
This is a basic procedure for high school students and useful for those who are new to molecular biology.
Bacteria Growth and Culture (1) and Bacteria Growth and Culture (2) (Michael Blaber)
Very useful background knowledge about bacteria growth and replication and very basic guide to bacteria culture techniques. If you are new to bacteria culture, it's a must to read this.
Streaking Bacterial Stocks (Donis Keller Lab)
Bacterial cells are streaked onto a medium to obtain an independent isolate. This is done to reduce the likelihood of working with a culture which has become contaminated and/or has accumulated mutations.
Replication and Preparation of Glycerol Stocks in 96 Well Plate

Bacteria Storage (Lazo Lab)
Maintenance of Probes in bacteria including Escherichia coli with stab agar or glycerol method
Bacterial cell maintenance (Roe, OU)
Basic procedures for bacteria culture
Long Term Storage of Bacterial Strains (Donis Keller Lab)
Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40% glycerol.
Lyophilizing Cells (Goldberg Lab)
Reusing Planting Plates (by Greg Stuart)
A protocol for cleaning and reusing the large 25 x 25 cm plates